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atra (1 mm in dmso)  (Millipore)

 
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    Structured Review

    Millipore atra (1 mm in dmso)
    ( A ) HL60 cells were treated for 7 days with solvent control, B/M alone and B/M combined with either 1 nM <t>ATRA,</t> 1 <t>nM</t> <t>1α,25(OH)</t> 2 vitamin D 3 (D 3 ) or both. A representative histogram of CD11b flow cytometry is shown in the left panel and results from N = 4 experiments in the middle panel . Mean is indicated by the black bar. ( B ) Cell morphology was analysed by Jenner-Giemsa staining of cytospins. Differentiated neutrophils are identified by classical poly-lobed nuclei (arrows) and apoptotic cells highlighted by asterix. Statistics: *p<0.001. ( C ) Primary AML cells taken from a karyotypically normal non-APL AML were treated with solvent CONT, B/M±1nmATRA/1nM D3. Differentiation was determined by CD11b staining and flow cytometry and by morphological analysis of Jenner-Giemsa stained cytospins after 18 days of treatment. ( D ) ROS generation was measured as described above in primary AML cells treated with cont, B/M±1nmATRA/1nM D3 for 48 hours.
    Atra (1 Mm In Dmso), supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/atra (1 mm in dmso)/product/Millipore
    Average 90 stars, based on 1 article reviews
    atra (1 mm in dmso) - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "Combined Bezafibrate and Medroxyprogesterone Acetate: Potential Novel Therapy for Acute Myeloid Leukaemia"

    Article Title: Combined Bezafibrate and Medroxyprogesterone Acetate: Potential Novel Therapy for Acute Myeloid Leukaemia

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0008147

    ( A ) HL60 cells were treated for 7 days with solvent control, B/M alone and B/M combined with either 1 nM ATRA, 1 nM 1α,25(OH) 2 vitamin D 3 (D 3 ) or both. A representative histogram of CD11b flow cytometry is shown in the left panel and results from N = 4 experiments in the middle panel . Mean is indicated by the black bar. ( B ) Cell morphology was analysed by Jenner-Giemsa staining of cytospins. Differentiated neutrophils are identified by classical poly-lobed nuclei (arrows) and apoptotic cells highlighted by asterix. Statistics: *p<0.001. ( C ) Primary AML cells taken from a karyotypically normal non-APL AML were treated with solvent CONT, B/M±1nmATRA/1nM D3. Differentiation was determined by CD11b staining and flow cytometry and by morphological analysis of Jenner-Giemsa stained cytospins after 18 days of treatment. ( D ) ROS generation was measured as described above in primary AML cells treated with cont, B/M±1nmATRA/1nM D3 for 48 hours.
    Figure Legend Snippet: ( A ) HL60 cells were treated for 7 days with solvent control, B/M alone and B/M combined with either 1 nM ATRA, 1 nM 1α,25(OH) 2 vitamin D 3 (D 3 ) or both. A representative histogram of CD11b flow cytometry is shown in the left panel and results from N = 4 experiments in the middle panel . Mean is indicated by the black bar. ( B ) Cell morphology was analysed by Jenner-Giemsa staining of cytospins. Differentiated neutrophils are identified by classical poly-lobed nuclei (arrows) and apoptotic cells highlighted by asterix. Statistics: *p<0.001. ( C ) Primary AML cells taken from a karyotypically normal non-APL AML were treated with solvent CONT, B/M±1nmATRA/1nM D3. Differentiation was determined by CD11b staining and flow cytometry and by morphological analysis of Jenner-Giemsa stained cytospins after 18 days of treatment. ( D ) ROS generation was measured as described above in primary AML cells treated with cont, B/M±1nmATRA/1nM D3 for 48 hours.

    Techniques Used: Flow Cytometry, Staining



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    atra (1 mm in dmso)  (Millipore)
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    Millipore atra (1 mm in dmso)
    ( A ) HL60 cells were treated for 7 days with solvent control, B/M alone and B/M combined with either 1 nM <t>ATRA,</t> 1 <t>nM</t> <t>1α,25(OH)</t> 2 vitamin D 3 (D 3 ) or both. A representative histogram of CD11b flow cytometry is shown in the left panel and results from N = 4 experiments in the middle panel . Mean is indicated by the black bar. ( B ) Cell morphology was analysed by Jenner-Giemsa staining of cytospins. Differentiated neutrophils are identified by classical poly-lobed nuclei (arrows) and apoptotic cells highlighted by asterix. Statistics: *p<0.001. ( C ) Primary AML cells taken from a karyotypically normal non-APL AML were treated with solvent CONT, B/M±1nmATRA/1nM D3. Differentiation was determined by CD11b staining and flow cytometry and by morphological analysis of Jenner-Giemsa stained cytospins after 18 days of treatment. ( D ) ROS generation was measured as described above in primary AML cells treated with cont, B/M±1nmATRA/1nM D3 for 48 hours.
    Atra (1 Mm In Dmso), supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/atra (1 mm in dmso)/product/Millipore
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    ( A ) HL60 cells were treated for 7 days with solvent control, B/M alone and B/M combined with either 1 nM <t>ATRA,</t> 1 <t>nM</t> <t>1α,25(OH)</t> 2 vitamin D 3 (D 3 ) or both. A representative histogram of CD11b flow cytometry is shown in the left panel and results from N = 4 experiments in the middle panel . Mean is indicated by the black bar. ( B ) Cell morphology was analysed by Jenner-Giemsa staining of cytospins. Differentiated neutrophils are identified by classical poly-lobed nuclei (arrows) and apoptotic cells highlighted by asterix. Statistics: *p<0.001. ( C ) Primary AML cells taken from a karyotypically normal non-APL AML were treated with solvent CONT, B/M±1nmATRA/1nM D3. Differentiation was determined by CD11b staining and flow cytometry and by morphological analysis of Jenner-Giemsa stained cytospins after 18 days of treatment. ( D ) ROS generation was measured as described above in primary AML cells treated with cont, B/M±1nmATRA/1nM D3 for 48 hours.
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    ( A ) HL60 cells were treated for 7 days with solvent control, B/M alone and B/M combined with either 1 nM <t>ATRA,</t> 1 <t>nM</t> <t>1α,25(OH)</t> 2 vitamin D 3 (D 3 ) or both. A representative histogram of CD11b flow cytometry is shown in the left panel and results from N = 4 experiments in the middle panel . Mean is indicated by the black bar. ( B ) Cell morphology was analysed by Jenner-Giemsa staining of cytospins. Differentiated neutrophils are identified by classical poly-lobed nuclei (arrows) and apoptotic cells highlighted by asterix. Statistics: *p<0.001. ( C ) Primary AML cells taken from a karyotypically normal non-APL AML were treated with solvent CONT, B/M±1nmATRA/1nM D3. Differentiation was determined by CD11b staining and flow cytometry and by morphological analysis of Jenner-Giemsa stained cytospins after 18 days of treatment. ( D ) ROS generation was measured as described above in primary AML cells treated with cont, B/M±1nmATRA/1nM D3 for 48 hours.
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    1 mm all-trans retinoic acid (atra)  (Millipore)
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    ( A ) HL60 cells were treated for 7 days with solvent control, B/M alone and B/M combined with either 1 nM <t>ATRA,</t> 1 <t>nM</t> <t>1α,25(OH)</t> 2 vitamin D 3 (D 3 ) or both. A representative histogram of CD11b flow cytometry is shown in the left panel and results from N = 4 experiments in the middle panel . Mean is indicated by the black bar. ( B ) Cell morphology was analysed by Jenner-Giemsa staining of cytospins. Differentiated neutrophils are identified by classical poly-lobed nuclei (arrows) and apoptotic cells highlighted by asterix. Statistics: *p<0.001. ( C ) Primary AML cells taken from a karyotypically normal non-APL AML were treated with solvent CONT, B/M±1nmATRA/1nM D3. Differentiation was determined by CD11b staining and flow cytometry and by morphological analysis of Jenner-Giemsa stained cytospins after 18 days of treatment. ( D ) ROS generation was measured as described above in primary AML cells treated with cont, B/M±1nmATRA/1nM D3 for 48 hours.
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    Image Search Results


    ( A ) HL60 cells were treated for 7 days with solvent control, B/M alone and B/M combined with either 1 nM ATRA, 1 nM 1α,25(OH) 2 vitamin D 3 (D 3 ) or both. A representative histogram of CD11b flow cytometry is shown in the left panel and results from N = 4 experiments in the middle panel . Mean is indicated by the black bar. ( B ) Cell morphology was analysed by Jenner-Giemsa staining of cytospins. Differentiated neutrophils are identified by classical poly-lobed nuclei (arrows) and apoptotic cells highlighted by asterix. Statistics: *p<0.001. ( C ) Primary AML cells taken from a karyotypically normal non-APL AML were treated with solvent CONT, B/M±1nmATRA/1nM D3. Differentiation was determined by CD11b staining and flow cytometry and by morphological analysis of Jenner-Giemsa stained cytospins after 18 days of treatment. ( D ) ROS generation was measured as described above in primary AML cells treated with cont, B/M±1nmATRA/1nM D3 for 48 hours.

    Journal: PLoS ONE

    Article Title: Combined Bezafibrate and Medroxyprogesterone Acetate: Potential Novel Therapy for Acute Myeloid Leukaemia

    doi: 10.1371/journal.pone.0008147

    Figure Lengend Snippet: ( A ) HL60 cells were treated for 7 days with solvent control, B/M alone and B/M combined with either 1 nM ATRA, 1 nM 1α,25(OH) 2 vitamin D 3 (D 3 ) or both. A representative histogram of CD11b flow cytometry is shown in the left panel and results from N = 4 experiments in the middle panel . Mean is indicated by the black bar. ( B ) Cell morphology was analysed by Jenner-Giemsa staining of cytospins. Differentiated neutrophils are identified by classical poly-lobed nuclei (arrows) and apoptotic cells highlighted by asterix. Statistics: *p<0.001. ( C ) Primary AML cells taken from a karyotypically normal non-APL AML were treated with solvent CONT, B/M±1nmATRA/1nM D3. Differentiation was determined by CD11b staining and flow cytometry and by morphological analysis of Jenner-Giemsa stained cytospins after 18 days of treatment. ( D ) ROS generation was measured as described above in primary AML cells treated with cont, B/M±1nmATRA/1nM D3 for 48 hours.

    Article Snippet: Bezafibrate (0.5 M in DMSO), MPA (5 mM in ethanol), ATRA (1 mM in DMSO), and 1α, 25(OH) 2 vitamin D 3 (1 mM in ethanol) (Sigma Aldrich).

    Techniques: Flow Cytometry, Staining